Comparative analysis of salt-induced changes in the root proteome of two accessions of the halophyte Cakile maritima

authored by

Ikram Belghith, Jennifer Senkler, Tatjana Hildebrandt, Chedly Abdelly, Hans Peter Braun, Ahmed Debez

Abstract

NaCl stress is a major abiotic stress factor limiting the productivity and the geographical distribution of many plant species. Although halophytes are able to withstand and even to require salt in the rhizosphere, roots are the most sensitive organs to salinity. Here, we investigate the variability of salt tolerance in two Tunisian accessions of the halophyte Cakile maritima (Raoued and Djerba, harvested from the semi-arid and arid Mediterranean bioclimatic stages, respectively) with a special emphasis on the proteomic changes in roots. Seedlings were hydroponically grown for one month under salt-free conditions and subsequently at three salinities (0, 100, and 300 mM NaCl). Physiological parameters (plant growth, water content, Na ⁺ , K ⁺ contents) and root protein profiles were analyzed. Plant biomass was higher in Raoued than in Djerba but the latter was impacted to a lesser extent by salinity, notably due to lower sodium accumulation and higher selectivity for K ⁺ . 121 and 97 salt-responsive proteins were identified in Djerba and Raoued accessions, respectively. These proteins can be assigned to several different functional categories: protein metabolism, nucleotide metabolism, amino acid metabolism, glutathione metabolism, translation and ribosome biogenesis, carbohydrate and energy metabolism, and reactive oxygen species regulation and detoxification. The comparative proteome analysis revealed that 33 proteins were salt-responsive in both accessions, while 88 and 64 proteins were salt-responsive only in the Djerba or Raoued accessions, respectively. Our results give deeper insights into the plasticity of salt-stress response of C. maritima in its native ecosystems.

Organisation(s)

Section Plant Molecular Biology and Plant Proteomics
Institute of Plant Genetics

External Organisation(s)

Center of Biotechnology of Borj Cedria (CBBC)
University of Tunis El-Manar

Type

Article

Journal

Plant physiology and biochemistry

Volume

130

Pages

20-29

No. of pages

10

ISSN

0981-9428

Publication date

21.06.2018

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Genetics, Plant Science

Sustainable Development Goals

SDG 15 - Life on Land

Electronic version(s)

https://doi.org/10.1016/j.plaphy.2018.06.029 (Access: Closed)