Expression of putative aquaporin genes in sweet cherry is higher in flesh than skin and most are downregulated during development

authored by

Yun Hao Chen, Bishnu P. Khanal, Marcus Linde, Thomas Debener, Merianne Alkio, Moritz Knoche

Abstract

Aquaporins (AQPs) function as gated membrane channels for water. They may have a role in water transport in sweet cherries (Prunus avium L.) where rain-induced cracking and the associated water uptake, severely limit production. The objectives were (1) to identify potential AQP genes in sweet cherry, (2) to establish their expression patterns during development and (3) to investigate their roles in the transmembrane movement of water at maturity. Based on an expression database of developing fruit and the recently published sweet cherry genome, 25 putative full length AQP genes were identified and 16 of them were expressed in the fruit. These 16 putative genes included seven PIPs, three TIPs, three NIPs and three SIPs. Most of them had six transmembrane domains and asparagine-proline-alanine motifs characteristic for AQPs. Expression differed among AQPs, tissues and developmental stages. The most highly expressed AQPs were PaPIP1;2, PaPIP1;4 and PaTIP1;1. Expression in the flesh generally exceeded that in the skin. Furthermore, expression decreased towards maturity. Incubating flesh discs in hypertonic sucrose solutions resulted in water efflux from the disc. HgCl₂ (1 mM), AgNO₃ (1 mM), H₂O₂ (100 mM) or Na acetate (100 mM) all decreased the rate of efflux, presumably through an inhibition of AQPs. Lower inhibitor concentrations had no significant effects. The results indicate: (1) that AQPs are expressed in sweet cherry fruits, (2) that expression is generally higher in the flesh than the skin and decreases towards maturity and (3) that AQPs are involved in water transport across plasma membranes.

Organisation(s)

Institute of Horticultural Production Systems
Institute of Plant Genetics

Type

Article

Journal

Scientia horticulturae

Volume

244

Pages

304-314

No. of pages

11

ISSN

0304-4238

Publication date

26.01.2019

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Horticulture

Electronic version(s)

https://doi.org/10.1016/j.scienta.2018.09.065 (Access: Closed)