The Use of RFLPs (Restriction Fragment Length Polymorphisms) Detects Germplasm Introgressions from Wild Species into Potato (Solanum tuberosum ssp. tuberosum) Breeding Lines

authored by

T. Debener, F. Salamini, Christiane Gebhardt

Abstract

The germplasm of the potato (S. tuberosum ssp. tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivated Solanum species of the Americas. In this paper we show that the Rflp analysis of potato‐breeding material can be used to detect chromosomal regions descended from more‐distantly related Solanum species. The Rflp patterns of individuals of ten Solanum species, ten breeding lines of S. tuberosum ssp. tuberosum and the cultivated variety ‘Bintje’ were analyzed. Rflp data for each of eight single loci of known genomic position were used for the computation of locus‐specific phenograms by distance matrix methods. Several of the potato‐breeding lines deviated clearly from the clustered species S. tuberosum, S. stenotomum and S. canasense for one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” chromosome fragments.

Organisation(s)

Section Molecular Plant Breeding

External Organisation(s)

Max Planck Institute for Plant Breeding Research

Type

Article

Journal

PLANT BREEDING

Volume

106

Pages

173-181

No. of pages

9

ISSN

0179-9541

Publication date

04.1991

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Agronomy and Crop Science, Genetics, Plant Science

Electronic version(s)

https://doi.org/10.1111/j.1439-0523.1991.tb00498.x (Access: Unknown)