Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)

authored by

Pamella Akoth Ogada, Leonard Muriithi Kiirika, Christin Lorenz, Jennifer Senkler, Hans Peter Braun, Hans Michael Poehling

Abstract

Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.

Organisation(s)

Section Plant Molecular Biology and Plant Proteomics
Institute of Plant Genetics

Type

Article

Journal

Developmental and Comparative Immunology

Volume

67

Pages

1-7

No. of pages

7

ISSN

0145-305X

Publication date

31.10.2016

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Immunology, Developmental Biology

Electronic version(s)

https://doi.org/10.15488/11653 (Access: Open)
https://doi.org/10.1016/j.dci.2016.10.013 (Access: Closed)