Analysis of the Rdr1 gene family in different Rosaceae genomes reveals an origin of an Rgene cluster after the split of Rubeae within the Rosoideae subfamily

authored by

Ina Menz, Deepika Lakhwani, Jérémy Clotault, Marcus Linde, Fabrice Foucher, Thomas Debener

Abstract

The Rdr1 gene confers resistance to black spot in roses and belongs to a large TNL gene family, which is organized in two major clusters at the distal end of chromosome 1. We used the recently available chromosome scale assemblies for the R. chinensis ‘Old Blush’ genome, re-sequencing data for nine rose species and genome data for Fragaria, Rubus, Malus and Prunus to identify Rdr1 homologs from different taxa within Rosaceae. Members of the Rdr1 gene family are organized into two major clusters in R. chinensis and at a syntenic location in the Fragaria genome. Phylogenetic analysis indicates that the two clusters existed prior to the split of Rosa and Fragaria and that one cluster has a more recent origin than the other. Genes belonging to cluster 2, such as the functional Rdr1 gene muRdr1A, were subject to a faster evolution than genes from cluster 1. As no Rdr1 homologs were found in syntenic positions for Prunus persica, Malus x domestica and Rubus occidentalis, a translocation of the Rdr1 clusters to the current positions probably happened after the Rubeae split from other groups within the Rosoideae approximately 70–80 million years ago during the Cretaceous period.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

University of Angers

Type

Article

Journal

PLOS ONE

Volume

15

ISSN

1932-6203

Publication date

23.01.2020

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Agricultural and Biological Sciences(all), General, Biochemistry, Genetics and Molecular Biology(all)

Electronic version(s)

https://doi.org/10.1371/journal.pone.0227428 (Access: Open)