Differential impact of amino acids on OXPHOS system activity following carbohydrate starvation in Arabidopsis cell suspensions

verfasst von

João Henrique F. Cavalcanti, Carla G.S. Quinhones, Peter Schertl, Danielle S. Brito, Holger Eubel, Tatjana Hildebrandt, Adriano Nunes-Nesi, Hans Peter Braun, Wagner L. Araújo

Abstract

Plant respiration mostly depends on the activity of glycolysis and the oxidation of organic acids in the tricarboxylic acid cycle to synthesize ATP. However, during stress situations plant cells also use amino acids as alternative substrates to donate electrons through the electron-transfer flavoprotein (ETF)/ETF:ubiquinone oxidoreductase (ETF/ETFQO) complex to the mitochondrial electron transport chain (mETC). Given this, we investigated changes of the oxidative phosphorylation (OXPHOS) system in Arabidopsis thaliana cell culture under carbohydrate starvation supplied with a range of amino acids. Induction of isovaleryl-CoA dehydrogenase (IVDH) activity was observed under carbohydrate starvation which was associated with increased amounts of IVDH protein detected by immunoblotting. Furthermore, activities of the protein complexes of the mETC were reduced under carbohydrate starvation. We also observed that OXPHOS system activity behavior is differently affected by different amino acids and that proteins associated with amino acids catabolism are upregulated in cells following carbohydrate starvation. Collectively, our results support the contention that ETF/ETFQO is an essential pathway to donate electrons to the mETC and that amino acids are alternative substrates to maintain respiration under carbohydrate starvation.

Organisationseinheit(en)

Institut für Pflanzengenetik

Externe Organisation(en)

Universidade Federal de Vicosa

Typ

Artikel

Journal

Physiologia plantarum

Band

161

Seiten

451-467

Anzahl der Seiten

17

ISSN

0031-9317

Publikationsdatum

02.08.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Physiologie, Genetik, Pflanzenkunde, Zellbiologie

Elektronische Version(en)

https://doi.org/10.1111/ppl.12612 (Zugang: Unbekannt)