Mitomycin C-induced effects on aerobic methanotrophs in a landfill cover soil

implications of a viral shunt?

verfasst von

Tanja Heffner, Thomas Kaupper, Mara Heinrichs, Hyo Jung Lee, Nadine Rüppel, Marcus A Horn, Adrian Ho

Abstract

A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.

Organisationseinheit(en)

Institut für Mikrobiologie

Externe Organisation(en)

Carl von Ossietzky Universität Oldenburg
Kunsan National University

Typ

Artikel

Journal

FEMS microbiology ecology

Band

99

ISSN

0168-6496

Publikationsdatum

06.2023

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Mikrobiologie, Ökologie, Angewandte Mikrobiologie und Biotechnologie

Elektronische Version(en)

https://doi.org/10.1093/femsec/fiad047 (Zugang: Geschlossen)