AFLP markers as a tool to reconstruct complex relationships

A case study in Rosa (Rosaceae)

verfasst von

Wim J.M. Koopman, Volker Wissemann, Katrien De Cock, Johan Van Huylenbroeck, Jan De Riek, Gerda J.H. Sabatino, Dirk Visser, Ben Vosman, Christiane M. Ritz, Bert Maes, Gun Werlemark, Hilde Nybom, Thomas Debener, Marcus Linde, Marinus J.M. Smulders

Abstract

The genus Rosa has a complex evolutionary history caused by several factors, often in conjunction: extensive hybridization, recent radiation, incomplete lineage sorting, and multiple events of polyploidy. We examined the applicability of AFLP markers for reconstructing (species) relationships in Rosa, using UPGMA clustering, Wagner parsimony, and Bayesian inference. All trees were well resolved, but many of the deeper branches were weakly supported. The cluster analysis showed that the rose cultivars can be separated into a European and an Oriental cluster, each being related to different wild species. The phylogenetic analyses showed that (1) two of the four subgenera (Hulthemia and Platyrhodon) do not deserve subgeneric status; (2) section Carolinae should be merged with sect. Cinnamomeae; (3) subsection Rubigineae is a monophyletic group within sect. Caninae, making sect. Caninae paraphyletic; and (4) there is little support for the distinction of the five other subsections within sect. Caninae. Comparison of the trees with morphological classifications and with previous molecular studies showed that all methods yielded reliable trees. Bayesian inference proved to be a useful alternative to parsimony analysis of AFLP data. Because of their genome-wide sampling, AFLPs are the markers of choice to reconstruct (species) relationships in evolutionary complex groups.

Organisationseinheit(en)

Institut für Pflanzengenetik
Abteilung Molekulare Pflanzenzüchtung

Externe Organisation(en)

Wageningen University and Research
Koopman Scientific Services
Justus-Liebig-Universität Gießen
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO)
Friedrich-Schiller-Universität Jena
Ecologisch Adviesbureau Maes
Swedish University of Agricultural Sciences

Typ

Artikel

Journal

American journal of botany

Band

95

Seiten

353-366

Anzahl der Seiten

14

ISSN

0002-9122

Publikationsdatum

03.2008

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Ökologie, Evolution, Verhaltenswissenschaften und Systematik, Genetik, Pflanzenkunde

Elektronische Version(en)

https://doi.org/10.3732/ajb.95.3.353 (Zugang: Unbekannt)