Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)

verfasst von

Pamella Akoth Ogada, Leonard Muriithi Kiirika, Christin Lorenz, Jennifer Senkler, Hans Peter Braun, Hans Michael Poehling

Abstract

Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection.

Organisationseinheit(en)

Abteilung Pflanzenmolekularbiologie und Pflanzenproteomik
Institut für Pflanzengenetik

Typ

Artikel

Journal

Developmental and Comparative Immunology

Band

67

Seiten

1-7

Anzahl der Seiten

7

ISSN

0145-305X

Publikationsdatum

01.02.2017

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Immunologie, Entwicklungsbiologie

Elektronische Version(en)

https://doi.org/10.1016/j.dci.2016.10.013 (Zugang: Geschlossen)
https://doi.org/10.15488/11653 (Zugang: Offen)