Effect of salt stress on aerobic methane oxidation and associated methanotrophs; a microcosm study of a natural community from a non-saline environment

verfasst von

Adrian Ho, Yongliang Mo, Hyo Jung Lee, Leopold Sauheitl, Zhongjun Jia, Marcus A. Horn

Abstract

We investigated the response of aerobic methane oxidation and the associated methanotrophs to salt-stress in a NaCl gradient ranging from 0 M (un-amended reference) to 0.6 M NaCl (seawater salinity) using a rice paddy soil as a model system. Salt-stress significantly inhibited methanotrophic activity at > 0.3 M NaCl; at 0.6 M NaCl amendment, methanotrophic activity fully ceased. MiSeq sequencing of the pmoA gene and group-specific qPCR analyses revealed that type Ia methanotroph (Methylobacter) appeared to be favored under salinity up to 0.3 M NaCl, increasing in numerical abundance, while the type Ib was adversely affected. This suggests niche differentiation within members of the gammaproteobacterial methanotrophs. Overall, rice paddy soil methanotrophs showed remarkable resistance to salt-stress.

Organisationseinheit(en)

Leibniz Forschungszentrum FZ:GEO
Institut für Mikrobiologie
AG Bodenchemie

Externe Organisation(en)

Chinese Academy of Sciences (CAS)
Kunsan National University

Typ

Artikel

Journal

Soil Biology and Biochemistry

Band

125

Seiten

210-214

Anzahl der Seiten

5

ISSN

0038-0717

Publikationsdatum

10.2018

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Mikrobiologie, Bodenkunde

Elektronische Version(en)

https://doi.org/10.15488/15939 (Zugang: Offen)
https://doi.org/10.1016/j.soilbio.2018.07.013 (Zugang: Geschlossen)