Recovery of Methanotrophic Activity Is Not Reflected in the Methane-Driven Interaction Network after Peat Mining

verfasst von

Thomas Kaupper, Lucas W. Mendes, Monica Harnisz, Sascha M.B. Krause, Marcus A. Horn, Adrian Ho

Abstract

Aerobic methanotrophs are crucial in ombrotrophic peatlands, driving the methane and nitrogen cycles. Peat mining adversely affects methanotrophs, but activity and community composition/abundances may recover after restoration. Considering that the methanotrophic activity and growth are significantly stimulated in the presence of other microorganisms, the methane-driven interaction network, which encompasses methanotrophs and nonmethanotrophs (i.e., the methanotrophic interactome), may also be relevant in conferring community resilience. Yet, little is known of the methanotrophic interactome's response to and recovery from disturbances. Here, we determined the recovery of the methanotrophic interactome as inferred by a co-occurrence network analysis comparing pristine and restored peatlands. We coupled a DNA-based stable isotope probing (SIP) approach using [13C]CH4 to a co-occurrence network analysis derived from the 13C-enriched 16S rRNA gene sequences to relate the response in methanotrophic activity to the structuring of the interaction network. Methanotrophic activity and abundances recovered after peat restoration since 2000. “Methylomonaceae” taxa were the predominantly active methanotrophs in both peatlands, but the peatlands differed in the relative abundances of Methylacidiphilaceae and Methylocystis. However, bacterial community compositions were distinct in both peatlands. Likewise, the methanotrophic interactome was profoundly altered in the restored peatland. Structuring of the interaction network after peat mining resulted in the loss of complexity and modularity, indicating a less connected and efficient network, which may have consequences in the event of recurring/future disturbances. Therefore, determining the response of the methane-driven interaction network, in addition to relating methanotrophic activity to community composition/abundances, provided a more comprehensive understanding of the resilience of the methanotrophs.

Organisationseinheit(en)

Institut für Mikrobiologie

Externe Organisation(en)

Universidade de Sao Paulo
University of Warmia and Mazury
East China Normal University

Typ

Artikel

Journal

Applied and Environmental Microbiology

Band

87

Anzahl der Seiten

13

ISSN

0099-2240

Publikationsdatum

12.02.2021

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Biotechnologie, Lebensmittelwissenschaften, Angewandte Mikrobiologie und Biotechnologie, Ökologie

Elektronische Version(en)

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8090869/ (Zugang: Offen)
https://doi.org/10.1128/aem.02355-20 (Zugang: Geschlossen)
https://doi.org/10.15488/15928 (Zugang: Offen)