Mitomycin C-induced effects on aerobic methanotrophs in a landfill cover soil

implications of a viral shunt?

verfasst von
Tanja Heffner, Thomas Kaupper, Mara Heinrichs, Hyo Jung Lee, Nadine Rüppel, Marcus A Horn, Adrian Ho
Abstract

A viral shunt can occur when phages going through a lytic cycle, including lysogenic phages triggered by inducing agents (e.g. mitomycin C), results in host lysis and the release of cell constituents and virions. The impact of a viral shunt on the carbon, including methane cycle in soil systems is poorly understood. Here, we determined the effects of mitomycin C on the aerobic methanotrophs in a landfill cover soil. To an extent, our results support a mitomycin C-induced viral shunt, as indicated by the significantly higher viral-like particle (VLP) counts relative to bacteria, elevated nutrient concentrations (ammonium, succinate), and initially impaired microbial activities (methane uptake and microbial respiration) after mitomycin C addition. The trend in microbial activities at <2 days largely corresponded to the expression of the pmoA and 16S rRNA genes. Thereafter (>11 days), the active bacterial community composition significantly diverged in the mitomycin C-supplemented incubations, suggesting the differential impact of mitomycin C on the bacterial community. Collectively, we provide insight on the effects of mitomycin C, and potentially a viral shunt, on the bacteria in the soil environment.

Organisationseinheit(en)
Institut für Mikrobiologie
Externe Organisation(en)
Carl von Ossietzky Universität Oldenburg
Kunsan National University
Typ
Artikel
Journal
FEMS microbiology ecology
Band
99
ISSN
0168-6496
Publikationsdatum
06.2023
Publikationsstatus
Veröffentlicht
Peer-reviewed
Ja
ASJC Scopus Sachgebiete
Mikrobiologie, Ökologie, Angewandte Mikrobiologie und Biotechnologie
Elektronische Version(en)
https://doi.org/10.1093/femsec/fiad047 (Zugang: Geschlossen)