An alternative model of the twin arginine translocation system

verfasst von

Thomas Brüser, Carsten Sanders

Abstract

The twin arginine translocation (Tat) system is a machinery which can translocate folded proteins across energy transducing membranes. Currently it is supposed that Tat substrates bind directly to Tat translocon components before a ΔpH-driven translocation occurs. In this review, an alternative model is presented which proposes that membrane integration could precede Tat-dependent translocation. This idea is mainly supported by the recent observations of Tat-independent membrane insertion of Tat substrates in vivo and in vitro. Membrane insertion may allow i) a quality control of the folded state by membrane bound proteases like FtsH, ii) the recognition of the membrane spanning signal peptide by Tat system components, and iii) a pulling mechanism of translocation. In some cases of folded Tat substrates, the membrane targeting process may require ATP-dependent N-terminal unfolding-steps.

Organisationseinheit(en)

Institut für Mikrobiologie

Externe Organisation(en)

Martin-Luther-Universität Halle-Wittenberg
University of Pennsylvania

Typ

Übersichtsarbeit

Journal

Microbiological Research

Band

158

Seiten

7-17

Anzahl der Seiten

11

ISSN

0944-5013

Publikationsdatum

2003

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Mikrobiologie

Elektronische Version(en)

https://doi.org/10.1078/0944-5013-00176 (Zugang: Offen)