Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP

verfasst von

Maria A. Schumacher, Mirka E. Wörmann, Max Henderson, Raul Salinas, Andreas Latoscha, Mahmoud M. Al-Bassam, Kumar Siddharth Singh, Elaine Barclay, Katrin Gunka, Natalia Tschowri

Abstract

Streptomyces are our principal source of antibiotics, which they generate concomitant with a complex developmental transition from vegetative hyphae to spores. c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. Here we show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link between c-di-GMP and glycogen metabolism in bacteria. Further, we show c-di-GMP binding is required for GlgX activity. We describe structures of apo and c-di-GMP-bound GlgX and, strikingly, their comparison shows c-di-GMP induces long-range conformational changes, reorganizing the catalytic pocket to an active state. Glycogen is an important glucose storage compound that enables animals to cope with starvation and stress. Our in vivo studies reveal the important biological role of GlgX in Streptomyces glucose availability control. Overall, we identify a function of c-di-GMP in controlling energy storage metabolism in bacteria, which is widespread in Actinobacteria.

Organisationseinheit(en)

Institut für Mikrobiologie

Externe Organisation(en)

Duke University
Humboldt-Universität zu Berlin
Bundesinstitut für Risikobewertung (BfR)
University of California at San Diego
John Innes Centre

Typ

Artikel

Journal

Nature Communications

Band

13

ISSN

2041-1723

Publikationsdatum

12.2022

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Chemie (insg.), Biochemie, Genetik und Molekularbiologie (insg.), Allgemein, Physik und Astronomie (insg.)

Elektronische Version(en)

https://doi.org/10.1038/s41467-022-33537-w (Zugang: Offen)