Research in the Tschowri group

Cellular Microbiology: Signalling in bacterial cell differentiation

Streptomyces are prominent natural ‘antibiotic factories’ that provide us with the majority of antibiotics used in medicine today.

In addition, they represent an exciting model to study cell differentiation and bacterial multicellularity. During their life cycle, they undergo a complex transition from filamentous, multicellular, vegetative hyphae to unicellular, dormant and highly resistant spores.

Natural product synthesis is genetically and temporally tightly connected to the developmental cycle of Streptomyces and both processes are regulated by multi-layered signalling cascades. In our lab, we apply molecular, biochemical and cell biological approaches to uncover the components and mechanisms of action of nucleotide-based signalling pathways in these complex Actinobacteria.

RESEARCH PROJECTS

Cyclic di-GMP signalling and regulation in bacterial cell differentiation

Cyclic di-GMP is a ubiquitous bacterial second messenger that is renowned for its function in guiding the transition between motility and sessility in most bacteria. In the non-motile streptomycetes, c-di-GMP is a key factor controlling the switch between their filamentous lifestyle and spore formation. Elevated levels of c-di-GMP bring the developmental program to a halt and arrest the cells in the vegetative, filamentous growth phase. Low levels of the cyclic dinucleotide significantly shorten the life cycle by enabling the vegetative hyphae to enter the sporulation programme in a precocious manner. We discovered that the master regulator of Streptomyces development, BldD, is a c-di-GMP-effector that mediates c-di-GMP signals. Binding of c-di-GMP to BldD induces protein dimerization and stimulates BldD-binding to target DNA leading to repression of sporulation genes.

Cellular levels of c-di-GMP are controlled by the competing activities of GGDEF-domain containing diguanylate cyclases (DGCs) that produce the molecule out of GTP, and by phosphodiesterases (PDEs) that carry an EAL or HD-GYP domain to degrade the second messenger. Streptomyces venezuelae, our main lab model, has 10 chromosomally-encoded GGDEF/EAL/HD-GYP genes. In our current projects, we focus on:

  • (I) dissecting the physiological functions of individual c-di-GMP enzymes in the differentiation of Streptomyces
  • (II) understanding of c-di-GMP patterns in time and space in multicellular Streptomyces 
  • (III) discovery on novel c-di-GMP effectors and functions in Streptomyces

Funding: Our research on c-di-GMP in Streptomyces is funded by the Emmy Noether Programme from the Deutsche Forschungsgemeinschaft (DFG).

Cyclic di-AMP enzymes, effectors and functions in Streptomyces

Cyclic di-AMP is mainly produced by Gram-positive bacteria and archaea. Its core function is to control cellular integrity by setting homeostasis of osmolytes that in many bacteria are used for osmoregulation. Changes in external osmolarity trigger water fluxes across the membrane, which can lead to cell dehydration or swelling and, finally, collapse or burst when osmobalance mechanisms fail to respond properly. As a key component of these mechanisms, c-di-AMP directly targets transport systems for osmoactive and osmoprotective substances such as potassium ions and low-molecular-weight compatible solutes in many bacteria.

The function of c-di-AMP in Streptomyces and other Actinobacteria is hardly understood. It was long unknown how the majority of Actinobacteria, stop c-di-AMP signals and which proteins bind the molecule to elicit cellular responses. We recently discovered the AtaC protein as a c-di-AMP-specific phosphodiesterase that is highly conserved in Actinobacteria and the RCK_C domain protein CpeA as a c-di-AMP effector. However, while we know that c-di-AMP in involved in regulation of osmotic stress and development in Streptomyces, the underlying mechanisms are unknown. Currently, we aim at addressing the following questions:

  • (I) What are the key components linking c-di-AMP signalling and developmental processes?
  • (II) How are osmostress and membrane integrity regulated in Streptomyces?
  • (III) Which signals trigger c-di-AMP dependent responses?

Funding: Our research on c-di-AMP in Streptomyces is funded by the SPP1879 Priority Programme from the Deutsche Forschungsgemeinschaft (DFG).

CONTACT

Prof. Dr. rer. nat. Natalia Tschowri
Address
Herrenhäuser Straße 2
30419 Hannover
Building
Room
133
Prof. Dr. rer. nat. Natalia Tschowri
Address
Herrenhäuser Straße 2
30419 Hannover
Building
Room
133