Depletion of the "gamma-type carbonic anhydrase-like" subunits of complex I affects central mitochondrial metabolism in Arabidopsis thaliana

Corrigendum to “depletion of the “gamma-type carbonic anhydrase-like” subunits of complex I affects central mitochondrial metabolism in Arabidopsis thaliana” (Biochimica et Biophysica Acta - Bioenergetics (2016) 1857(1) (60–71))

authored by

Steffanie Fromm, Jennifer Göing, Christin Lorenz, Christoph Peterhänsel, Hans Peter Braun

Abstract

"Gamma-type carbonic anhydrase-like" (CAL) proteins form part of complex I in plants. Together with "gamma carbonic anhydrase" (CA) proteins they form an extra domain which is attached to the membrane arm of complex I on its matrix exposed side. In Arabidopsis two CAL and three CA proteins are present, termed CAL1, CAL2, CA1, CA2 and CA3. It has been proposed that the carbonic anhydrase domain of complex I is involved in a process mediating efficient recycling of mitochondrial CO₂ for photosynthetic carbon fixation which is especially important during growth conditions causing increased photorespiration. Depletion of CAL proteins has been shown to significantly affect plant development and photomorphogenesis. To better understand CAL function in plants we here investigated effects of CAL depletion on the mitochondrial compartment. In mutant lines and cell cultures complex I amount was reduced by 90-95% but levels of complexes III and V were unchanged. At the same time, some of the CA transcripts were less abundant. Proteome analysis of CAL depleted cells revealed significant reduction of complex I subunits as well as proteins associated with photorespiration, but increased amounts of proteins participating in amino acid catabolism and stress response reactions. Developmental delay of the mutants was slightly alleviated if plants were cultivated at high CO₂. Profiling of selected metabolites revealed defined changes in intermediates of the citric acid cycle and amino acid catabolism. It is concluded that CAL proteins are essential for complex I assembly and that CAL depletion specifically affects central mitochondrial metabolism.

Organisation(s)

Institute of Botany
Institute of Plant Genetics

Type

Article

Journal

Biochimica et Biophysica Acta - Bioenergetics

Volume

1857

Pages

60-71

No. of pages

12

ISSN

0005-2728

Publication date

19.10.2015

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biophysics, Biochemistry, Cell Biology

Electronic version(s)

https://doi.org/10.1016/j.bbabio.2015.10.006 (Access: Open)
https://doi.org/10.1016/j.bbabio.2016.07.001 (Access: Unknown)