Genomic evidence for two pathways of formaldehyde oxidation and denitrification capabilities of the species Paracoccus methylovorus sp. nov.

authored by

Timsy Timsy, Undine Behrendt, Andreas Ulrich, Bärbel U. Foesel, Tobias Spanner, Meina Neumann-Schaal, Jacqueline Wolf, Michael Schloter, Marcus A. Horn, Steffen Kolb

Abstract

Three strains (H4-D09T, S2-D11 and S9-F39) of a member of the genus Paracoccus attributed to a novel species were isolated from topsoil of temperate grasslands. The genome sequence of the type strain H4-D09T exhibited a complete set of genes required for denitrification as well as methylotrophy. The genome of H4-D09T included genes for two alternative pathways of formaldehyde oxidation. Besides the genes for the canonical glutathione (GSH)-dependent formaldehyde oxidation pathway, all genes for the tetrahydrofolate-formaldehyde oxidation pathway were identified. The strain has the potential to utilize methanol and/or methylamine as a single carbon source as evidenced by the presence of methanol dehydrogenase (mxaFI) and methylamine dehydrogenase (mau) genes. Apart from dissimilatory denitrification genes (narA, nirS, norBC and nosZ), genes for assimilatory nitrate (nasA) and nitrite reductases (nirBD) were also identified. The results of phylogenetic analysis based on 16S rRNA genes coupled with riboprinting revealed that all three strains represented the same species of genus Paracoccus. Core genome phylogeny of the type strain H4-D09T indicated that Paracoccus thiocyanatus and Paracoccus denitrificans are the closest phylogenetic neighbours. The average nucleotide index (ANI) and digital DNA-DNA hybridization (dDDH) with the closest phylogenetic neighbours revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. The major respiratory quinone is Q-10, and the predominant cellular fatty acids are C18 : 1ω7c, C19 : 0cyclo ω7c, and C16 : 0, which correspond to those detected in other members of the genus. The polar lipid profile consists of a diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), aminolipid (AL), glycolipid (GL) and an unidentified lipid (L).On the basis of our results, we concluded that the investigated isolates represent a novel species of the genus Paracoccus, for which the name Paracoccus methylovorus sp. nov. (type strain H4-D09T=LMG 31941T= DSM 111585T) is proposed.

Organisation(s)

Institute of Microbiology

External Organisation(s)

Leibniz Centre for Agricultural Landscape Research (ZALF)
Humboldt-Universität zu Berlin
Helmholtz Zentrum München - German Research Center for Environmental Health
Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures

Type

Article

Journal

International Journal of Systematic and Evolutionary Microbiology

Volume

72

ISSN

1466-5026

Publication date

21.10.2022

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Microbiology, Ecology, Evolution, Behavior and Systematics

Electronic version(s)

https://doi.org/10.1099/ijsem.0.005581 (Access: Closed)