Protoplast isolation and plant regeneration of different genotypes of Petunia and calibrachoa

verfasst von

L. Meyer, M. Serek, T. Winkelmann

Abstract

Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

Organisationseinheit(en)

Abteilung Reproduktion und Entwicklung

Typ

Artikel

Journal

Plant Cell, Tissue and Organ Culture

Band

99

Seiten

27-34

Anzahl der Seiten

8

ISSN

0167-6857

Publikationsdatum

08.08.2009

Publikationsstatus

Veröffentlicht

Peer-reviewed

Ja

ASJC Scopus Sachgebiete

Gartenbau

Elektronische Version(en)

https://doi.org/10.1007/s11240-009-9572-4 (Zugang: Geschlossen)