Protoplast isolation and plant regeneration of different genotypes of Petunia and calibrachoa

authored by

L. Meyer, M. Serek, T. Winkelmann

Abstract

Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.

Organisation(s)

Section Reproduction and Development

Type

Article

Journal

Plant Cell, Tissue and Organ Culture

Volume

99

Pages

27-34

No. of pages

8

ISSN

0167-6857

Publication date

08.08.2009

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Horticulture

Electronic version(s)

https://doi.org/10.1007/s11240-009-9572-4 (Access: Closed)