Development of markers for shoot organogenesis in roses

authored by
Thi Hong Nguyen, Traud Winkelmann, Thomas Debener
Abstract

Shoot organogenesis is an essential step for genetic engineering and the study of developmental processes in roses. Several adventitious shoot regeneration protocols have been established, but the number of responding cultivars is very limited. Furthermore, large differences in regeneration capacity between genotypes were reported rendering many cultivars as recalcitrant for shoot regeneration. Therefore, knowledge about the genetic complexity of the capacity for adventitious shoot regeneration and genes influencing this trait would be helpful for optimising regeneration protocols. Previously we analysed an association panel of 96 genotypes for in vitro shoot regeneration from petioles, and we detected a number of markers associated with the phenotypic traits. Here we present results of experiments to verify significantly associated markers in independent populations of garden roses using the KASP (Kompetitive Allele Specific PCR) technology.

Organisation(s)
Institute of Horticultural Production Systems
Section Molecular Plant Breeding
Type
Article
Journal
Acta Horticulturae
Volume
1232
Pages
7-14
No. of pages
8
ISSN
0567-7572
Publication date
01.02.2019
Publication status
Published
Peer reviewed
Yes
ASJC Scopus subject areas
Horticulture
Electronic version(s)
https://doi.org/10.17660/ActaHortic.2019.1232.2 (Access: Closed)