TALE and TALEN genome editing technologies

authored by

Sebastian Becker, Jens Boch

Abstract

TALEN were the first easy-to-use genome editing technology and sparked the genome editing revolution. Their application in multiple species brought targeted mutagenesis to the attention of scientists worldwide. Key breakthrough successes of genome editing have since been achieved using TALEN, among these, the first commercialization of an edited crop and the first human cured from cancer. TALEN have since been largely replaced by the CRISPR technologies which are somewhat easier to build, much easier to multiplex, and have spawned multiple derived techniques. Nevertheless, the flexible and precise positioning of TALEN is unmatched, and thus they have continued to evolve to new functionalities. Here, we assemble essential facts, design guidelines as well as important past and exciting novel developments.

Organisation(s)

Section Plant Biotechnology

Type

Article

Journal

Gene and Genome Editing

Volume

2

ISSN

2666-3880

Publication date

12.2021

Publication status

Published

Peer reviewed

Yes

Electronic version(s)

https://doi.org/10.1016/j.ggedit.2021.100007 (Access: Open)