Genetic analysis of adventitious root formation in vivo and in vitro in a diversity panel of roses

authored by
Thi Hong Nhung Nguyen, Sophie Tänzer, Jasmin Rudeck, Traud Winkelmann, Thomas Debener

In a diversity panel of 95 rose genotypes, we induced adventitious root formation under both in vitro and in vivo conditions and performed a genome-wide association study to analyse rooting performance using genotype information from the 68 K Axiom WagRhSNP chip. For each tested condition, three independent experiments were carried out. Significant variations in in vitro root number (ranging from 0.12–18.7) and total root length (0.26–25.76 cm) as well as in vivo root number, root length and root biomass were recorded among the genotypes. For the in vitro parameters, we found 49 SNPs that were significantly associated with in vitro root length, whereas the other rooting parameters did not exhibit any significant associations. For the in vivo parameters, we found 98 SNPs associated with root number, 218 SNPs associated with root length and 4 SNPs associated with root biomass. Some of these SNPs were located in genes with homology to root-related genes such as the WUSCHEL-related homeobox 8-like and ETHYLENE INSENSITIVE 3-like, which were associated with in vitro root length, and auxin response factor 19, protein AUXIN RESPONSE 4, and a MYC2 transcription factor (AtMYC2), which were associated with in vivo root number and root length. We mapped the SNPs to the recently published high-quality rose genome sequence and detected several regions in the genome that harbour additional homologues of genes known to be related to rooting traits in other species, such as SCARECROW (SCR) on chromosome 3 and WUSCHEL-related homeobox genes on chromosomes 1, 4 and 6. These markers will serve as the starting point for future experiments to validate the genes in other populations and examine their functionality in transgenic experiments.

Institute of Horticultural Production Systems
Section Molecular Plant Breeding
External Organisation(s)
Vietnam Academy of Agricultural Sciences (Vaas)
Scientia horticulturae
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Peer reviewed
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