Comparative Analyses of Protein Complexes by Blue Native DIGE

authored by

Katrin Peters, Hans Peter Braun

Abstract

Classically, DIGE is carried out on the basis of two-dimensional (2D) IEF/SDS PAGE. This allows comparative analyses of large protein sets. However, 2D IEF/SDS PAGE only poorly resolves hydrophobic proteins and is not compatible with native protein characterizations. Blue native PAGE represents a powerful alternative. Combined with CyDye labeling, blue native DIGE offers several useful applications like quantitative comparison of protein complexes of related protein fractions. Here we present a protocol for fluorophore labeling of native protein fractions for separation by blue native PAGE.

Organisation(s)

Institute of Plant Genetics

Type

Contribution to book/anthology

Pages

145-154

No. of pages

10

Publication date

12.01.2012

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Molecular Biology, Genetics

Electronic version(s)

https://doi.org/10.1007/978-1-61779-573-2_10 (Access: Unknown)