The carbonic anhydrase domain of plant mitochondrial complex I

authored by
Steffanie Fromm, Jennifer Senkler, Eduardo Zabaleta, Christoph Peterhänsel, Hans Peter Braun
Abstract

The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma-type carbonic anhydrases. The Arabidopsis genome codes for five complex I-integrated gamma-type carbonic anhydrases (γCA1, γCA2, γCA3, γCAL1, γCAL2), but only three copies of this group of proteins form an individual extra domain. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1/γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA/γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.

Organisation(s)
Section Plant Molecular Biology and Plant Proteomics
Institute of Botany
Institute of Plant Genetics
External Organisation(s)
Universidad Nacional de Mar del Plata
Type
Review article
Journal
Physiologia plantarum
Volume
157
Pages
289-296
No. of pages
8
ISSN
0031-9317
Publication date
21.06.2016
Publication status
Published
Peer reviewed
Yes
ASJC Scopus subject areas
Physiology, Genetics, Plant Science, Cell Biology
Electronic version(s)
https://doi.org/10.15488/11655 (Access: Open)
https://doi.org/10.1111/ppl.12424 (Access: Closed)