Development of the WagRhSNP AXIOM SNP array based on sequences from tetraploid cut roses and garden roses

authored by

M. J.M. Smulders, R. E. Voorrips, G. D. Esselink, T. M. Santos Leonardo, W. P.C. Vant' Westende, M. Vukosavljev, C. F.S. Koning-Boucoiran, W. E. Van De Weg, P. Arens, D. Schulz, T. Debener, L. Bellon, M. Mittmann, A. Pirani, T. Webster, F. Brew, P. Cox, C. Maliepaard

Abstract

Rose, as many other important ornamental, vegetable and field crops, is polyploid. This poses constraints in genetic analyses due to the occurrence of multiple alleles at marker and trait loci and the existence of multiple allele dosages. Developments in marker discovery (next generation sequencing), detection (SNP arrays) and analysis (software for dosage scoring) now make it feasible to develop high-density molecular marker maps for the homologous chromosomes in tetraploids separately, and thus perform QTL analysis in tetraploids. We developed a SNP array for rose to develop genetic maps in tetraploid garden roses and cut roses, which are to be used for inheritance studies and genetic mapping. Here we have indicated the general strategy followed for developing a SNP array and for scoring and using the SNP data generated, and elaborated on the activities undertaken to use the WagRhSNP Axiom array in rose. The array design is not proprietary but can be used by all researchers working in rose.

Organisation(s)

Institute of Plant Genetics
Section Molecular Plant Breeding

External Organisation(s)

Wageningen University and Research
Thermo Fisher Scientific
Roath B.V.

Type

Conference contribution

Pages

177-184

No. of pages

8

Publication date

25.01.2015

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Horticulture

Electronic version(s)

https://doi.org/10.17660/ActaHortic.2015.1064.20 (Access: Unknown)