Pseudomonas campi sp. nov., a nitrate-reducing bacterium isolated from grassland soil

authored by: Timsy, Tobias Spanner, Andreas Ulrich, Susanne Kublik, Bärbel U. Foesel, Steffen Kolb, Marcus A. Horn, Undine Behrendt
Abstract: A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2^T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2^T=LMG 31521^T=DSM 110222^T) is proposed.
Organisation(s): Institute of Microbiology
External Organisation(s): Leibniz Centre for Agricultural Landscape Research (ZALF)
Helmholtz Zentrum München - German Research Center for Environmental Health
Type: Article
Journal: International Journal of Systematic and Evolutionary Microbiology
Volume: 71
ISSN: 1466-5026
Publication date: 05.05.2021
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Microbiology, Ecology, Evolution, Behavior and Systematics
Electronic version(s): https://doi.org/10.15488/15943 (Access: Open)
https://doi.org/10.1099/ijsem.0.004799 (Access: Closed)