Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability

authored by

Kathleen Prinz, Stephan Schie, Thomas Debener, Isabell Hensen, Kurt Weising

Abstract

We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.

Organisation(s)

Institute of Plant Genetics
Section Molecular Plant Breeding

External Organisation(s)

University of Kassel
Martin Luther University Halle-Wittenberg

Type

Article

Journal

Molecular ecology resources

Volume

9

Pages

1424-1426

No. of pages

3

ISSN

1755-098X

Publication date

09.2009

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Biotechnology, Ecology, Evolution, Behavior and Systematics, Genetics

Electronic version(s)

https://doi.org/10.1111/j.1755-0998.2009.02680.x (Access: Unknown)