Early manganese-toxicity response in Vigna unguiculata L.
A proteomic and transcriptomic study
- authored by
- Hendrik Führs, Moritz Hartwig, Laura Elisa Buitrago Molina, Dimitri Heintz, Alain Van Dorsselaer, Hans Peter Braun, Walter Johannes Horst
- Abstract
The apoplast is known to play a predominant role in the expression of manganese (Mn) toxicity in cowpea (Vigna unguiculata L.) leaves. To unravel early Mn-toxicity responses after 1-3 days Mn treatment also in the leaf symplast, we studied the symplastic reactions induced by Mn in two cultivars differing in Mn tolerance on a total cellular level. Comparative proteome analyses of plants exposed to low or high Mn allowed to identify proteins specifically affected by Mn, particularly in the Mn-sensitive cowpea cultivar. These proteins are involved in CO2 fixation, stabilization of the Mn cluster of the photosystem II, pathogenesis-response reactions and protein degradation. Chloroplastic proteins important for CO2 fixation and photosynthesis were of lower abundance upon Mn stress suggesting scavenging of metabolic energy for a specific stress response. Transcriptome analyses supported these findings, but additionally revealed an upregulation of genes involved in signal transduction only in the Mn-sensitive cultivar. In conclusion, a coordinated interplay of apoplastic and symplastic reactions seems to be important during the Mn-stress response in cowpea.
- Organisation(s)
-
Institute of Plant Genetics
Institute of Plant Nutrition
- External Organisation(s)
-
University of Strasbourg
- Type
- Article
- Journal
- PROTEOMICS
- Volume
- 8
- Pages
- 149-159
- No. of pages
- 11
- ISSN
- 1615-9853
- Publication date
- 19.12.2007
- Publication status
- Published
- Peer reviewed
- Yes
- ASJC Scopus subject areas
- Biochemistry, Molecular Biology
- Electronic version(s)
-
https://doi.org/10.15488/11664 (Access:
Open)
https://doi.org/10.1002/pmic.200700478 (Access: Unknown)
https://onlinelibrary.wiley.com/doi/epdf/10.1002/pmic.200700478 (Access: Unknown)
