The role of amino acid metabolism during abiotic stress release

authored by

Willian Batista-Silva, Björn Heinemann, Nils Rugen, Adriano Nunes-Nesi, Wagner L. Araújo, Hans Peter Braun, Tatjana M. Hildebrandt

Abstract

Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.

Organisation(s)

Institute of Plant Genetics

External Organisation(s)

Universidade Federal de Vicosa

Type

Article

Journal

Plant Cell and Environment

Volume

42

Pages

1630-1644

No. of pages

15

ISSN

0140-7791

Publication date

12.04.2019

Publication status

Published

Peer reviewed

Yes

ASJC Scopus subject areas

Physiology, Plant Science

Electronic version(s)

https://doi.org/10.1111/pce.13518 (Access: Closed)