Complexome Profiling Reveals Association of PPR Proteins with Ribosomes in the Mitochondria of Plants

authored by: Nils Rugen, Henryk Straube, Linda E. Franken, Hans Peter Braun, Holger Eubel
Abstract: Mitochondrial transcripts are subject to a wealth of processing mechanisms including cis- and trans-splicing events, as well as base modifications (RNA editing). HUndreds of proteins are required for these processes in plant mitochondria, many of which belong to the pentatricopeptide repeat (PPR) protein superfamily. The structure, localization, and function of these proteins is only poorly Understood. Here we present evidence that several PPR proteins are boUnd to mitoribosomes in plants. A novel complexome profiling strategy in combination with chemical crosslinking has been employed to systematically define the protein constituents of the large and the small ribosomal subunits in the mitochondria of plants. We identified more than 80 ribosomal proteins, which include several PPR proteins and other non-conventional ribosomal proteins. These findings reveal a potential coupling of transcriptional and translational events in the mitochondria of plants. Furthermore, the data indicate an extremely high molecular mass of the “small” subunit, even exceeding that of the “large” subunit.
Organisation(s): Institute of Plant Genetics
External Organisation(s): Heinrich Pette Institute - Leibniz Institute for Experimental Virology (HPI)
Type: Article
Journal: Molecular and Cellular Proteomics
Volume: 18
Pages: 1345-1362
No. of pages: 18
ISSN: 1535-9476
Publication date: 07.2019
Publication status: Published
Peer reviewed: Yes
ASJC Scopus subject areas: Analytical Chemistry, Biochemistry, Molecular Biology
Electronic version(s): https://doi.org/10.1074/mcp.RA119.001396 (Access: Open)
https://doi.org/10.15488/11680 (Access: Open)
https://doi.org/10.15488/12222 (Access: Open)
https://doi.org/10.1074/mcp.AAC119.001674 (Access: Open)